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- Wake Forest NC, USA
Hi all, I think that is probably unlikely in mainland Europe, they tend to be a lot more careful with potentially toxic metals than we are in the UK (or I dare say you are in the USA).
What are you talking about? Our President says we have the cleanest greenest nation on earth! Our environment is so clean we don't even need environmental regulations anymore! We're BIGLY green!
Another thought was that if the water is aged with peat etc then any metal ions will be complexed and much less available.I do this, but I suspect the OP's rain-water is too soft. cheers Darrel
Yes, that would be a problem. He may need to add some soil or Ca supplement to keep the Daphnia alive long enough to test for toxicity. There are some kinds of small Daphnids that i've seen in soft, acidic vernal pools in the NC Sandhills and Coastal plain region, but I don't know what they are.