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No, new blood in all of that time. In 2012 I collected and brought home 3 specimens of each sex. I got 2 breeding pairs and a lot of offspring. These were crossed with the 2 unpaired fish and with different broods. All bred true to the wild form for about 6 generations (~5 years). Then I would find find an occasional fish that showed 2 lateral spots, or only 1 on 1 side, instead of the one like on the holotype and my original fish. These were never used for breeding. Yes I still get some with 2 spots but with proper breeding the fish I use for brood stock conform to the wild form. I wonder (if I were unethical) if I could sell the 2-spot A. wolli as A. payaminonis for big money.