Again, why 24 hours? It depends on which preparation you get. If it says 48 hours in the instructions, stick to the instructions. The instructions will also say to remove it after the treatment, usually with a 80% waterchange and using active carbon for 48 hours.
Use plastic plants and still add leaves to offer them cover, even though you don't add sand. The leaves are expendable and the plants can be disinfected.Can the fish survive 28 days in a quarantine tank with RO water + medication, without substrate/sand and without leaves (in an empty tank)???
Sorry for my perhaps silly questions, and thank you for your patience with me.