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Yes. Females can be very territorial with other females (or their reflection). With that said, the flaring from the females is usually more face on/open mouth/gill flaring in my experience
Kinda depends on the temperament of the parents… I’ve had fish that were totally fine with 3-4 generations of fry with them, and others that would clear the table before every spawn.
Trying to catch fry sucks. I’ve always preferred to move the pair to a new tank, and wait till the kids were...
I don’t have the slightest idea what the exact problem is, but with ammonia and nitrite reading anything higher than 0 I’d be inclined to change a bunch of water right now, change a bunch of water tomorrow, and probably do the same on Monday before throwing random meds/fixes at the tank.
1&2 both look girly to me. 3 looks male. 4…I’m not sure if it’s a beat down male trying to look female, or actually female. With the Icana fish, the males will develop quite a bit of that rusty red in the face, while the females will carry the rusty red color on the flanks ( but not face)
A sp D6/mitu, A sp D37/keimenfleck, A sp D39. All fantastic Apistogramma that wonderful looking, fairly rare in the hobby, and quite a bit more wide ranging in terms of water chemistry than one might be led to believe.
Interesting. Maybe she decided it was time to be a he. Would be super interesting to see what happens if you introduced/forced a juvie female with the old female
Water chemistry is perfect. Tank size is not… In my experience, I’d say the tank is smallish for one pair, with two pairs I’m sort of amazed you’ve avoided any murders. Move a pair, I’d wager the results improve wildly without the pressure of the second pair
I’ve never made much of an effort to do anything special with fry in the tank. Seems to me a fish that would be hatched in the rainy season wouldn’t be too bothered by swings in temperature.